THE BACTERIOCHLOROPHYLL BIOSYNTHESIS GENE, BCHM, OF RHODOBACTER-SPHAEROIDES ENCODES S-ADENOSYL-L-METHIONINE - MG PROTOPORPHYRIN-IX METHYLTRANSFERASE

被引：42

作者：

GIBSON, LCD

HUNTER, CN

机构：

[1] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,ROBERT HILL INST PHOTOSYNTH,SHEFFIELD S10 2UH,ENGLAND

[2] UNIV SHEFFIELD,DEPT MOLEC BIOL & BIOTECHNOL,KREBS INST BIOMOLEC RES,SHEFFIELD S10 2UH,ENGLAND

来源：

FEBS LETTERS | 1994年 / 352卷 / 02期

关键词：

PHOTOSYNTHESIS; BACTERIOCHLOROPHYLL; CHLOROPHYLL; RHODOBACTER SPHAEROIDES; METHYLTRANSFERASE;

D O I：

10.1016/0014-5793(94)00934-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The identity of this product was verified by HPLC. The bchM gene product is therefore assigned to the methyltransferase step in bacteriochlorophyll biosynthesis.

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页码：127 / 130

页数：4

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