THE USE OF CALCIUM ALGINATE GELS FOR SOLIDS SEPARATION AND DIFFUSIONAL CHROMATOGRAPHY OF BIOLOGICAL-MATERIALS

被引：10

作者：

KIERSTAN, M

机构：

[1] Institute for Industrial Research and Standards Biochemical Unit, University College, Galway, Ireland

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1981年 / 23卷 / 04期

关键词：

Alcohol dehydrogenase - Calcium alginate - Calcium alginate gels - Chloride solutions - Model system - Solids separation - Yeast extracts;

D O I：

10.1002/bit.260230405

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of calcium alginate gels for the Solids Separation techniques was demonstrated by entrapment of a yeast extract in calcium alginate pellets and the study of the release of alcohol dehydrogenase and NADH into a dilute calcium chloride solution. The use of calcium alginate gels for a Diffusional Chromatography technique was demonstrated in a model system by a fractionation of NAD and hemoglobin release following their entrapment in calcium alginate pellets. The advantages of these techniques and their potentials are discussed. © 1981 John Wiley & Sons, Inc.

引用

页码：707 / 716

页数：10

共 5 条

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[2] PHYSICAL STUDIES ON CELL IMMOBILIZATION USING CALCIUM ALGINATE GELS
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[3] PHYSICAL STUDIES ON THE MECHANICAL STABILITY OF COLUMNS OF CALCIUM ALGINATE GEL PELLETS CONTAINING ENTRAPPED MICROBIAL-CELLS
CHEETHAM, PSJ
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1979, 1 (03) : 183 - 188
[4] KERSTERS K, 1966, METHOD ENZYMOL, V9, P346
[5] IMMOBILIZATION OF MICROBIAL-CELLS, SUBCELLULAR ORGANELLES, AND ENZYMES IN CALCIUM ALGINATE GELS
KIERSTAN, M
BUCKE, C
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1977, 19 (03) : 387 - 397

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