THE REPLICATOR REGION OF THE RHIZOBIUM-LEGUMINOSARUM CRYPTIC PLASMID PRL8JI

被引：33

作者：

TURNER, SL ^{[1
]}

YOUNG, JPW ^{[1
]}

机构：

[1] UNIV YORK, DEPT BIOL, YORK YO1 5YW, N YORKSHIRE, ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1995年 / 133卷 / 1-2期

关键词：

RHIZOBIUM LEGUMINOSARUM; PLASMID REPLICATION; PARTITION FUNCTION; REPA; REPB; REPC;

D O I：

10.1111/j.1574-6968.1995.tb07860.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The replicator region of the cryptic plasmid pRL8JI from Rhizobium leguminosarum strain 3841 was cloned and sequenced. The recombinant plasmid (pYK3) was selected by function from a partial EcoRI library of total DNA cloned in pSUP202 and shows incompatibility with plasmid pRL8JI when conjugated into R. leguminosarum strains 3841 and its derivative 1062. The cloned insert(approximate to 10.5 kb) comprises five EcoRI fragments none of which confers replicative stability when cloned individually. A single 5.0-kb BamHI fragment, that spans all five EcoRI fragments and confers replicative stability on pSUP202 in R. leguminosarum, has been sequenced. This replicator region shows organisational and sequence similarity to the replicator regions of the Agrobacterium plasmids pTiB6S3 and pRiA4b. It has three open reading frames (repA, repB, repC) and a conserved intergenic sequence.

引用

页码：53 / 58

页数：6

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