COPURIFICATION OF PROTEASES WITH BASIC FIBROBLAST GROWTH-FACTOR (FGF)

被引：5

作者：

HO, PL ^{[1
]}

CARPENTER, MR ^{[1
]}

SMILLIE, LB ^{[1
]}

GAMBARINI, AG ^{[1
]}

机构：

[1] UNIV ALBERTA, DEPT BIOCHEM, MRC, PROT STRUCT & FUNCT GRP, EDMONTON T6G 2H7, ALBERTA, CANADA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92157-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acidic and basic fibroblast growth factors (FGFs) are proteins of 16-18 kDa. Other forms of 25-30 kDa related to this growth factor family have recently been described. All these components bind tightly to heparin-Sepharose, a property that allows the purification of several FGF-related proteins. During the purification of acidic and basic FGFs from bovine pituitary glands, we detected the presence of 28-30 kDa components that are immunoreactive against antibasic FGF antisera. However, microsequencing analysis revealed that the 28-30 kDa components are lysosomal proteases that co-elute with basic FGF from heparin-Sepharose columns. The involvement of these proteases in the etiology of microheterogenous forms of FGFs and/or release of FGFs from the extracellular matrix is discussed. © 1990.

引用

页码：769 / 774

页数：6

共 22 条

[1] AN ONCOGENE ISOLATED BY TRANSFECTION OF KAPOSIS-SARCOMA DNA ENCODES A GROWTH-FACTOR THAT IS A MEMBER OF THE FGF FAMILY [J].

BOVI, PD ;

CURATOLA, AM ;

KERN, FG ;

GRECO, A ;

ITTMANN, M ;

BASILICO, C .

CELL, 1987, 50 (05) :729-737

[2] THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].

BURGESS, WH ;

MACIAG, T .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606

[3]

BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5

[4] PROCESSING, SECRETION, AND BIOLOGICAL PROPERTIES OF A NOVEL GROWTH-FACTOR OF THE FIBROBLAST GROWTH-FACTOR FAMILY WITH ONCOGENIC POTENTIAL [J].

DELLIBOVI, P ;

CURATOLA, AM ;

NEWMAN, KM ;

SATO, Y ;

MOSCATELLI, D ;

HEWICK, RM ;

RIFKIN, DB ;

BASILICO, C .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (07) :2933-2941

[5] POTENTIAL ONCOGENE PRODUCT RELATED TO GROWTH-FACTORS [J].

DICKSON, C ;

PETERS, G .

NATURE, 1987, 326 (6116) :833-833

[6] PURIFICATION AND AMINO-ACID-SEQUENCE OF CHICKEN LIVER CATHEPSIN-L [J].

DUFOUR, E ;

OBLED, A ;

VALIN, C ;

BECHET, D ;

RIBADEAUDUMAS, B ;

HUET, JC .

BIOCHEMISTRY, 1987, 26 (18) :5689-5695

[7] BIOSYNTHESIS OF LYSOSOMAL ENDOPEPTIDASES [J].

ERICKSON, AH .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1989, 40 (01) :31-41

[8] MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF FIBROBLAST GROWTH-FACTOR, AN ANGIOGENIC FACTOR WHICH ALSO CONTROLS THE PROLIFERATION AND DIFFERENTIATION OF MESODERM AND NEUROECTODERM DERIVED CELLS [J].

GOSPODAROWICZ, D ;

NEUFELD, G ;

SCHWEIGERER, L .

CELL DIFFERENTIATION, 1986, 19 (01) :1-17

[9]

HO PL, 1988, BIOCHEM INT, V17, P973

[10] MULTIPLE FORMS OF BASIC FIBROBLAST GROWTH-FACTOR - AMINO-TERMINAL CLEAVAGES BY TUMOR CELL-DERIVED AND BRAIN CELL-DERIVED ACID PROTEINASES [J].

KLAGSBRUN, M ;

SMITH, S ;

SULLIVAN, R ;

SHING, Y ;

DAVIDSON, S ;

SMITH, JA ;

SASSE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1839-1843

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