IDENTIFICATION OF A LOW-MOLECULAR-WEIGHT FORM OF EPITHELIAL TRANSFORMING GROWTH-FACTOR

被引：5

作者：

DUNNINGTON, D

PRICHETT, W

MOYER, M

GREIG, R

机构：

[1] SmithKline Beecham Pharmaceuticals, Department of Cell Sciences, King of Prussia, PA 19406-2799

来源：

LIFE SCIENCES | 1990年 / 47卷 / 22期

关键词：

D O I：

10.1016/0024-3205(90)90441-S

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We have purified a novel form epithelial transforming growth factor (TGFe) from bovine kidney. Acid ethanol extracts of kidney were fractionated by size exclusion, reverse phase and cation exchange chromatography and activity was monitored by measuring growth of SW13 adrenocortical carcinoma cells in soft agar. The purified material was highly cationic, bound weakly to heparin and gave a band at 13-15000 Mr by SDS-PAGE following Bolton-Hunter iodination. This band corresponded to the migration of biological activity extractable from gel slices. The results suggest that we have isolated a truncated form of TGFe which nonetheless retains biological activity. © 1990.

引用

页码：2059 / 2063

页数：5

共 6 条

[1] AUTOMATION OF DATA ACQUISITION AND PROCESSING IN ASSAYS FOR ANCHORAGE-INDEPENDENT GROWTH - APPLICATION TO THE PURIFICATION OF EPITHELIAL TRANSFORMING GROWTH-FACTOR
DUNNINGTON, DJ
PINSKY, S
MATTES, D
PRICHETT, W
EARL, CQ
GREIG, R
ANZANO, MA
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) : 257 - 264
[2] HALPER J, 1983, CANCER RES, V43, P1972
[3] HALPER J, 1987, CANCER RES, V47, P4552
[4] LOBB R, 1986, J BIOL CHEM, V261, P1924
[5] PURIFICATION OF TRANSFORMING GROWTH-FACTOR TYPE-E
PARNELL, PG
WUNDERLICH, J
CARTER, B
HALPER, J
[J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1990, 42 (02) : 111 - 116
[6] STABILIZING BASIC FIBROBLAST GROWTH-FACTOR USING PROTEIN ENGINEERING
SENO, M
SASADA, R
IWANE, M
SUDO, K
KUROKAWA, T
ITO, K
IGARASHI, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 151 (02) : 701 - 708

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