INDIRECT ELECTROCHEMICAL DETERMINATION OF L-TYROSINE USING MUSHROOM TYROSINASE IN SOLUTION

被引：49

作者：

RIVAS, GA ^{[1
]}

SOLIS, VM ^{[1
]}

机构：

[1] NATL UNIV CORDOBA,FAC CIENCIAS QUIM,INST INVEST FISICOQUIM CORDOBA,DEPT FISICOQUIM,SUC 16,CC 61,RA-5016 CORDOBA,ARGENTINA

来源：

ANALYTICAL CHEMISTRY | 1991年 / 63卷 / 23期

关键词：

D O I：

10.1021/ac00023a018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electroanalytical method for quantifying L-tyrosine is described. This technique is based on the measurement of the reduction current of dopachrome, which is formed in solution as a product of the oxidation of L-tyrosine by oxygen catalyzed by the enzyme mushroom tyrosinase also present in solution. The effect of ascorbic acid as cofactor is analyzed and optimal experimental conditions are determined. A vitreous carbon electrode activated in alkaline solution was employed. The method proposed is a new application of the use of tyrosinase for the determination of tyrosine using voltammetric techniques and is an alternative to the amperometric tyrosine determination technique.

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页码：2762 / 2765

页数：4

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