AN OXYGEN-EVOLVING PHOTOSYSTEM-II COMPLEX ASSOCIATED WITH THE CORE SUBSTRUCTURE OF THE PHYCOBILISOME FROM SYNECHOCOCCUS-ELONGATUS

被引：8

作者：

KASHINO, Y

ENAMI, I

IGARASHI, S

KATOH, S

机构：

[1] SCI UNIV TOKYO,FAC SCI,DEPT BIOL,SHINJUKU KU,TOKYO 162,JAPAN

[2] UNIV TOKYO,FAC SCI,DEPT BIOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

PLANT AND CELL PHYSIOLOGY | 1992年 / 33卷 / 03期

关键词：

CROSS-LINKING; LARGE LINKER POLYPEPTIDE; PHOTOSYSTEM-II; PHYCOBILISOME CORE; POLYPEPTIDE COMPOSITION; SYNECHOCOCCUS-ELONGATUS;

D O I：

10.1093/oxfordjournals.pcp.a078249

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Oxygen-evolving photosystem II (PS II) particles isolated from the thermophilic cyanobacterium Synechococcus elongatus consisted of about twenty polypeptides. Six polypeptides were identified by reaction with specific antisera as constituent subunit polypeptides of oxygen-evolving PS II reaction center complexes. The most abundant polypeptides were the a and beta subunits of allophycocyanin. Comparison with the polypeptide profile of isolated phycobilisomes, as well as immunoblotting with an antiserum against the large linker polypeptide, showed that the large linker polypeptide or some proteolytic fragments of it were present in the preparation. Thus, each PS II particle is, in essence, an oxygen-evolving PS II complex that is associated with the core substructure of the phycobilisome. Cross-linking experiments indicated that fragments of the large linker polypeptides are closely associated with one another and that the Chl-carrying 47-kDa polypeptide is located in close proximity to the D2 protein and the extrinsic 33-kDa protein.

引用

页码：259 / 266

页数：8

共 35 条

[1] CLOSE ASSOCIATION OF THE 33-KDA EXTRINSIC PROTEIN WITH THE APOPROTEIN OF CPA1 IN PHOTOSYSTEM-II [J].