EVALUATION OF A GENETICALLY-ENGINEERED CELL-LINE AND A HISTOCHEMICAL BETA-GALACTOSIDASE ASSAY TO DETECT HERPES-SIMPLEX VIRUS IN CLINICAL SPECIMENS

被引：31

作者：

STABELL, EC ^{[1
]}

OROURKE, SR ^{[1
]}

STORCH, GA ^{[1
]}

OLIVO, PD ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,CLIN VIROL LAB,ST LOUIS,MO 63110

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 10期

关键词：

D O I：

10.1128/JCM.31.10.2796-2798.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel histochemical method was compared with a cytopathic effect (CPE) assay for the identification of herpes simplex virus (HSV) in clinical specimens. The method utilizes a stably transformed cell line (BHKICP6LacZ-5) that expresses beta-galactosidase only after infection with HSV. A total of 96 specimens submitted to our diagnostic virology laboratory were analyzed. Thirty-one specimens contained HSV as evidenced by positive CPE, and all were positive for beta-galactosidase staining. CPE were not evident for 2 or more days in 15 of the 31 positive specimens, whereas the histochemical stain was positive in all 31 positives by 16 to 24 h. This preliminary study shows that the BHKICP6LacZ-5 cell line can be used in a rapid, sensitive, and specific assay for the detection of HSV in clinical specimens.

引用

页码：2796 / 2798

页数：3

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