PHOTOSYNTHESIS AND WATER RELATIONS IN TRANSGENIC TOBACCO PLANTS WITH T-DNA CARRYING GENE 4 FOR CYTOKININ SYNTHESIS

被引：15

作者：

CATSKY, J

POSPISILOVA, J

MACHACKOVA, I

WILHELMOVA, N

SESTAK, Z

机构：

[1] Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha 6, 160 0

来源：

BIOLOGIA PLANTARUM | 1993年 / 35卷 / 03期

关键词：

D O I：

10.1007/BF02928516

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Expression of the gene 4 for cytokinin synthesis in two clones of transgenic tobacco plants resulted in slightly higher (ca. 10 - 20 %) levels of endogenous cytokinins - zeatin, zeatin riboside and isopentenyladenosine. This was associated with changes in photosynthetic processes on chloroplast and leaf level. In comparison with non-transformed plantlets a higher degree of grana stacking was found in both clones of transgenic plants which indicated a higher proportion of photosystem 2 light-harvesting complex in thylakoids. This was supported by a decreased chlorophyll a/b ratio in the separated grana fraction. The rate of leaf net photosynthetic rate (measured as CO2 uptake) was ca. 25 % higher in both clones than in non-transformed plants. Also the rates of dark and light respiration, and CO2 compensation concentration were higher in transgenic plants. On the other hand, parameters of water relations (water, osmotic and pressure potentials, stomatal and cuticular transpiration rates) were not significantly affected in transgenic tobacco plants.

引用

页码：393 / 399

页数：7

共 11 条

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