STRUCTURE OF GAMMA-CHYMOTRYPSIN AT 5.5 A RESOLUTION

被引：34

作者：

COHEN, GH

SILVERTON, EW

MATTHEWS, BW

BRAXTON, H

DAVIES, DR

机构：

[1] Laboratory of Molecular Biology National Institute of Arthritis, Metabolic Diseases National Institutes of Health Bethesda

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 44卷 / 01期

关键词：

D O I：

10.1016/0022-2836(69)90409-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An electron density map at 5.5 Å resolution has been computed for γ-chymotrypsin inhibited with toluenesulfonyl fluoride. Data were collected for the native enzyme crystals, for the inhibited enzyme tosyl-γCHT† † Abbreviations used: CHT, chymotrypsin; tosyl-F, toluenesulfonyl fluoride; pipsyl-F, p-iodobenzenesulfonyl fluoride; PMMCMBS-, p-methoxy-m-chloromercuribenzenesulfonyl-; PMS-, phenylmethanesulfonyl. and for four heavy-atom derivatives prepared by soaking the crystals in p-iodophenylsulfonyl fluoride, p-methoxy-m-chloromercuribenzenesulfonyl fluoride, KI3 and K2HgI4. The shape of the molecule has been determined and the position of the active center located. The γCHT molecule has been compared with αCHT. © 1969.

引用

页码：129 / +

页数：1

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