CHARACTERIZATION OF PROTEIN S, A GAMMA-CARBOXYGLUTAMIC ACID CONTAINING PROTEIN FROM BOVINE AND HUMAN-PLASMA

被引：253

作者：

DISCIPIO, RG ^{[1
]}

DAVIE, EW ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT BIOCHEM, SEATTLE, WA 98195 USA

来源：

BIOCHEMISTRY | 1979年 / 18卷 / 05期

关键词：

D O I：

10.1021/bi00572a026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein S is a vitamin K dependent protein of unknown function, which is present in mammalian plasma. It was isolated from bovine plasma by barium citrate adsorption and elution, ammonium sulfate fractionation, and column chromatography on DEAE-Sephadex, heparin-agarose, and polyhomoarginine-Sepharose. Bovine Protein S (Mr 64 200) is a single-chain glycoprotein with an amino-terminal sequence of Ala-Asn-Thr-Leu-Leu-. It contains 7.0% carbohydrate and 10 residues of γ-carboxyglutamic acid per mol of protein. Human Protein S (Mr 69 000) is also a single-chain glycoprotein with an amino-terminal sequence of Ala-AsnSer-Leu-Leu-. It contains 7.8% carbohydrate and 10 residues of 7-carboxyglutamic acid per mol of protein. These results indicate that Protein S from bovine or human plasma shows many similarities to the other vitamin K dependent proteins present in plasma. © 1979, American Chemical Society. All rights reserved.

引用

页码：899 / 904

页数：6

共 57 条

[1] MEASUREMENT OF PROTEIN CONCENTRATION WITH INTERENCES OPTICS [J].