A QUANTITATIVE MODEL FOR THE MECHANISM OF ACTION OF THE CYTOCHROME-C PEROXIDASE OF PSEUDOMONAS-AERUGINOSA

被引：28

作者：

FOOTE, N ^{[1
]}

TURNER, R ^{[1
]}

BRITTAIN, T ^{[1
]}

GREENWOOD, C ^{[1
]}

机构：

[1] UNIV E ANGLIA,SCH BIOL SCI,NORWICH NR4 7TJ,NORFOLK,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1992年 / 283卷

关键词：

D O I：

10.1042/bj2830839

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Each of the elementary reaction steps in both the activation process and catalytic cycle of the cytochrome c peroxidase of Pseudomonas aeruginosa was characterized using stopped-flow methods. A synthesis of these data led to the establishment of a quantitative model for the action of this enzyme. Comparisons were made between experimental data and calculations over a wide range of enzyme, reductant and H2O2 concentrations. Close agreement was found between empirical and simulated reaction time courses from millisecond to tens of seconds time ranges, giving us confidence in the validity of the quantitative model of this enzyme's actions.

引用

页码：839 / 843

页数：5

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