A SEMIQUANTITATIVE CYTOCHROME P450IA1 ELISA - A SIMPLE METHOD FOR STUDYING THE MONOOXYGENASE INDUCTION RESPONSE IN ENVIRONMENTAL MONITORING AND ECOTOXICOLOGICAL TESTING OF FISH

被引：110

作者：

GOKSOYR, A

机构：

[1] Department of Biochemistry, University of Bergen, N-5009 Bergen

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 1991年 / 101卷 / 03期

关键词：

D O I：

10.1016/0048-9697(91)90038-G

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) for detection of the aromatic and chlorinated hydrocarbon-inducible cytochrome P450IA1 isozyme in fish samples is presented. The method can be utilized in monitoring programs for screening large series of samples simultaneously, and may be especially suited to show induction responses in samples where catalytic measurement is difficult or impossible due to (a) small sample or organ size, (b) loss of activities in bad storage conditions, or (c) inhibiting compounds present in the sample.

引用

页码：255 / 262

页数：8

共 17 条

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GOKSOYR, A
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[6] SPECIES CHARACTERISTICS OF THE HEPATIC XENOBIOTIC AND STEROID BIOTRANSFORMATION SYSTEMS OF 2 TELEOST FISH, ATLANTIC COD (GADUS-MORHUA) AND RAINBOW-TROUT (SALMO-GAIRDNERI)
GOKSOYR, A
ANDERSSON, T
HANSSON, T
KLUNGSOYR, J
ZHANG, Y
FORLIN, L
[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY, 1987, 89 (03) : 347 - 360
[7] PURIFICATION OF HEPATIC-MICROSOMAL CYTOCHROMES-P-450 FROM BETA-NAPHTHOFLAVONE-TREATED ATLANTIC COD (GADUS-MORHUA), A MARINE TELEOST FISH
GOKSOYR, A
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 840 (03) : 409 - 417
[8] RESPONSE OF COD (GADUS-MORHUA) LARVAE AND JUVENILES TO OIL EXPOSURE DETECTED WITH ANTI-COD CYTOCHROME-P-450C IGG AND ANTI-SCUP CYTOCHROME-P-450E MAB 1-12-3
GOKSOYR, A
SERIGSTAD, B
SOLBERG, TS
STEGEMAN, JJ
[J]. MARINE ENVIRONMENTAL RESEARCH, 1988, 24 (1-4) : 31 - 35
[9] GOKSOYR A, 1989, CYTOCHROME P-450 : BIOCHEMISTRY AND BIOPHYSICS /, P694
[10] GOKSOYR A, 1991, IN PRESS FISH PHYSL

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