EXPRESSION OF SOME HEPATOCYTE-LIKE FUNCTIONAL-PROPERTIES OF WRL-68 CELLS IN CULTURE

被引：38

作者：

GUTIERREZRUIZ, MC ^{[1
]}

BUCIO, L ^{[1
]}

SOUZA, V ^{[1
]}

GOMEZ, JJ ^{[1
]}

CAMPOS, C ^{[1
]}

CARABEZ, A ^{[1
]}

机构：

[1] UNIV NACL AUTONOMA MEXICO,FAC MED,DEPT HISTOL,MEXICO CITY,DF,MEXICO

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 1994年 / 30A卷 / 06期

关键词：

CELL CULTURE; HUMAN FETUS; HEPATIC CELL LINE; WRL-68; CELLS;

D O I：

10.1007/BF02634356

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Some morphologic and functional characteristics of an hepatic fetal human epithelial cell line (WRL-68 cells) were determined to validate the use of these cells as an in vitro hepatic model. WRL-68 cells have a morphologic structure similar to hepatocytes and hepatic primary cultures. They secrete alpha-fete protein and albumin and exhibit a cytokeratin pattern similar to other hepatic cultures. WRL-68 cells preserve the activity of some characteristic or specific liver enzymes or both used in clinical chemistry for the diagnosis of hepatic disorders, i.e. alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase, and alkaline phosphatase.

引用

页码：366 / 371

页数：6

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