QUANTIFICATION OF ABSCISIC-ACID USING LIQUID-CHROMATOGRAPHY THERMOSPRAY IONIZATION TANDEM MASS-SPECTROMETRY FOR SELECTED REACTION MONITORING

被引：7

作者：

OKAMOTO, M

TAKAHASHI, K

DOI, T

机构：

[1] Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd, Osaka, 554, 1–98, 3‐chome, Kasugade‐naka, Konohana‐ku

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1993年 / 7卷 / 12期

关键词：

D O I：

10.1002/rcm.1290071202

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatography/thermospray ionization tandem mass spectrometry (LC/TSP-MS/MS) method was developed for the quantification of abscisic acid (ABA) in crude plant extracts without derivatization. By this technique interfering chemical background ions are eliminated and a detection limit for ABA of 0.2ng is achieved. Reproducibility for quantification is attained by parallel reaction monitoring of ABA and added ABA-d6 used as an internal standard. The standard curve is linear over the range 200 pg to 10 ng of injected ABA. As examples of the application of the methodology, the quantification of ABA in some plant tissues is given.

引用

页码：1067 / 1069

页数：3

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