SINGLE-CHANNEL BEHAVIOR OF RECOMBINANT BETA(2) GAP JUNCTION CONNEXONS RECONSTITUTED INTO PLANAR LIPID BILAYERS

被引：35

作者：

BUEHLER, LK ^{[1
]}

STAUFFER, KA ^{[1
]}

GILULA, NB ^{[1
]}

KUMAR, NM ^{[1
]}

机构：

[1] MRC, MOLEC BIOL LAB, CAMBRIDGE, ENGLAND

来源：

BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 05期

关键词：

D O I：

10.1016/S0006-3495(95)80353-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The beta(2) gap junction protein (Cx26) was expressed in an insect cell line by infection with a baculovirus vector containing the rat beta(2) cDNA. Isolated beta(2) gap junction connexons were reconstituted into planar lipid bilayers. Single channel activity was observed with a unitary conductance of 35-45 pS in 200 mM KCl. Channels with conductance values of 60 pS and 90-110 pS also coexisted with the lower conducting channel suggesting that there are channels with different conductance properties within a population of connexons. Channel activity was observed at voltages of up to 150 mV. Furthermore, the characterization of these channel properties from the beta(2) connexons that were generated by this heterologous expression system has provided the basis for identifying an endogenous beta(2) connexon channel in material reconstituted from native rat liver gap junctions.

引用

页码：1767 / 1775

页数：9

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