CLONING AND EXPRESSION OF A CDNA COVERING THE COMPLETE CODING REGION OF THE P-32 SUBUNIT OF HUMAN PRE-MESSENGER-RNA SPLICING FACTOR-SF2

被引：54

作者：

HONORE, B

MADSEN, P

RASMUSSEN, HH

VANDEKERCKHOVE, J

CELIS, JE

LEFFERS, H

机构：

[1] AARHUS UNIV,DANISH CTR HUMAN GENOME RES,DK-8000 AARHUS C,DENMARK

[2] STATE UNIV GHENT,PHYSIOL CHEM LAB,B-9000 GHENT,BELGIUM

来源：

GENE | 1993年 / 134卷 / 02期

关键词：

RECOMBINANT DNA; VACCINIA VIRUS EXPRESSION; TRANSFORMATION-SENSITIVE PROTEIN;

D O I：

10.1016/0378-1119(93)90108-F

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and expressed a cDNA encoding the 32-kDa subunit (P32) of the human pre-mRNA splicing factor, SF2. This cDNA extends beyond the 5'-end of a previously reported cDNA [Krainer et al., Cell 66 (1991) 383-394]. Importantly, our fragment includes an ATG start codon which was absent from the previously reported cDNA, where it was suggested that translation might initiate at a CTG codon instead of at an ATG codon. Using the vaccinia virus (Vv) expression system, we demonstrate that translation starts at the conventional ATG start codon and not at the CTG codon. The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa.

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页码：283 / 287

页数：5

相关论文

共 17 条

[1] CLONING AND SEQUENCE OF THE HUMAN NUCLEAR-PROTEIN CYCLIN - HOMOLOGY WITH DNA-BINDING PROTEINS [J].

ALMENDRAL, JM ;

HUEBSCH, D ;

BLUNDELL, PA ;

MACDONALDBRAVO, H ;

BRAVO, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (06) :1575-1579

[2] ALTERATIONS IN THE PHENOTYPE OF PLANT-CELLS STUDIED BY NH2-TERMINAL AMINO-ACID-SEQUENCE ANALYSIS OF PROTEINS ELECTROBLOTTED FROM TWO-DIMENSIONAL GEL-SEPARATED TOTAL EXTRACTS [J].

BAUW, G ;

DELOOSE, M ;

INZE, D ;

VANMONTAGU, M ;

VANDEKERCKHOVE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4806-4810

[3] PROTEIN-ELECTROBLOTTING AND PROTEIN-MICROSEQUENCING STRATEGIES IN GENERATING PROTEIN DATA-BASES FROM TWO-DIMENSIONAL GELS - (COMPUTERIZED PROTEIN DATA-BASES HUMAN GENOME SEQUENCING) [J].

BAUW, G ;

VANDAMME, J ;

PUYPE, M ;

VANDEKERCKHOVE, J ;

GESSER, B ;

RATZ, GP ;

LAURIDSEN, JB ;

CELIS, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (20) :7701-7705

[4] EUKARYOTIC START AND STOP TRANSLATION SITES [J].

CAVENER, DR ;

RAY, SC .

NUCLEIC ACIDS RESEARCH, 1991, 19 (12) :3185-3192

[5] A COMPREHENSIVE 2-DIMENSIONAL GEL PROTEIN DATABASE OF NONCULTURED UNFRACTIONATED NORMAL HUMAN EPIDERMAL-KERATINOCYTES - TOWARDS AN INTEGRATED APPROACH TO THE STUDY OF CELL-PROLIFERATION, DIFFERENTIATION AND SKIN DISEASES [J].

CELIS, JE ;

MADSEN, P ;

RASMUSSEN, HH ;

LEFFERS, H ;

HONORE, B ;

GESSER, B ;

DEJGAARD, K ;

OLSEN, E ;

MAGNUSSON, N ;

KIIL, J ;

CELIS, A ;

LAURIDSEN, JB ;

BASSE, B ;

RATZ, GP ;

ANDERSEN, AH ;

WALBUM, E ;

BRANDSTRUP, B ;

PEDERSEN, PS ;

BRANDT, NJ ;

PUYPE, M ;

VANDAMME, J ;

VANDEKERCKHOVE, J .

ELECTROPHORESIS, 1991, 12 (11) :802-872

[6] THE MRC-5 HUMAN EMBRYONAL LUNG FIBROBLAST 2-DIMENSIONAL GEL CELLULAR PROTEIN DATABASE - QUANTITATIVE IDENTIFICATION OF POLYPEPTIDES WHOSE RELATIVE ABUNDANCE DIFFERS BETWEEN QUIESCENT, PROLIFERATING AND SV40-TRANSFORMED CELLS [J].

CELIS, JE ;

DEJGAARD, K ;

MADSEN, P ;

LEFFERS, H ;

GESSER, B ;

HONORE, B ;

RASMUSSEN, HH ;

OLSEN, E ;

LAURIDSEN, JB ;

RATZ, G ;

MOURITZEN, S ;

BASSE, B ;

HELLERUP, M ;

CELIS, A ;

PUYPE, M ;

VANDAMME, J ;

VANDEKERCKHOVE, J .

ELECTROPHORESIS, 1990, 11 (12) :1072-+

[7]

HONORE B, 1992, J BIOL CHEM, V267, P8485

[8] THE THERMAL-STABILITY OF OLIGONUCLEOTIDE DUPLEXES IS SEQUENCE INDEPENDENT IN TETRAALKYLAMMONIUM SALT-SOLUTIONS - APPLICATION TO IDENTIFYING RECOMBINANT DNA CLONES [J].

JACOBS, KA ;

RUDERSDORF, R ;

NEILL, SD ;

DOUGHERTY, JP ;

BROWN, EL ;

FRITSCH, EF .

NUCLEIC ACIDS RESEARCH, 1988, 16 (10) :4637-4650

[9] FUNCTIONAL EXPRESSION OF CLONED HUMAN SPLICING FACTOR SF2 - HOMOLOGY TO RNA-BINDING PROTEINS, U1-70K, AND DROSOPHILA SPLICING REGULATORS [J].

KRAINER, AR ;

MAYEDA, A ;

KOZAK, D ;

BINNS, G .

CELL, 1991, 66 (02) :383-394

[10] MOLECULAR-CLONING, OCCURRENCE, AND EXPRESSION OF A NOVEL PARTIALLY SECRETED PROTEIN PSORIASIN THAT IS HIGHLY UP-REGULATED IN PSORIATIC SKIN [J].

MADSEN, P ;

RASMUSSEN, HH ;

LEFFERS, H ;

HONORE, B ;

DEJGAARD, K ;

OLSEN, E ;

KIIL, J ;

WALBUM, E ;

ANDERSEN, AH ;

BASSE, B ;

LAURIDSEN, JB ;

RATZ, GP ;

CELIS, A ;

VANDEKERCKHOVE, J ;

CELIS, JE .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (04) :701-712