CLONING, NUCLEOTIDE SEQUENCING, AND EXPRESSION OF AN OPINE DEHYDROGENASE GENE FROM ARTHROBACTER SP STRAIN 1C

被引：25

作者：

DAIRI, T ^{[1
]}

ASANO, Y ^{[1
]}

机构：

[1] TOYAMA PREFECTURAL UNIV, BIOTECHNOL RES CTR, TOYAMA 93903, JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1995年 / 61卷 / 08期

关键词：

D O I：

10.1128/AEM.61.8.3169-3171.1995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD(+)-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).

引用

页码：3169 / 3171

页数：3

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