PARASITE GLYCOCONJUGATES .3. SYNTHESIS OF SUBSTRATE-ANALOGS OF EARLY INTERMEDIATES IN THE BIOSYNTHETIC-PATHWAY OF GLYCOSYLPHOSPHATIDYLINOSITOL MEMBRANE ANCHORS

Substrate analogues of sodium 1D-6-0-(2-[H-3(3)]acetamido-2-deoxy-alpha-D-glucopyranosyl)-myo-inositol 1-[sn-2,3-bis(palmitoyloxy)propyl phosphate] 3, including the lipid-depleted compounds 9, 11 and 15, have been prepared for biological evaluation with a partially purified de-N-acetylase from the bloodstream form of the parasitic protozoan Trypanosoma brucei. Such analogues as 2-deoxy-alpha-D-Glcp-PI25 and alpha-D-Glcp-PI33 have also been prepared and tested as potential inhibitors of this de-N-acetylase.