EFFECT OF THE INITIAL POTENTIAL RAMP ON THE ACCURACY OF ELECTROPHORETIC MOBILITIES IN CAPILLARY ELECTROPHORESIS

被引：30

作者：

WILLIAMS, BA ^{[1
]}

VIGH, G ^{[1
]}

机构：

[1] TEXAS A&M UNIV, DEPT CHEM, COLLEGE STN, TX 77843 USA

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 17期

关键词：

D O I：

10.1021/ac00113a051

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In several commercial capillary electrophoretic instruments, the potential is increased linearly at the beginning of a separation from zero to the programmed value. The ramp time can be as long as 20 s, resulting in grossly distorted mobility values when the migration times are commensurable with the ramp times. An expression is proposed to correct for the effects of the reduced field strength during the initial potential ramp.

引用

收藏

页码：3079 / 3081

页数：3

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