ISOLATION OF ANTIBODIES TO C-TERMINAL HEPTAPEPTIDE OF MYOGLOBIN WITH A SYNTHETIC PEPTIDE

被引:21
作者
GIVAS, J
CENTENO, ER
MANNING, M
SEHON, AH
机构
[1] Departments of Chemistry and Biochemistry, McGill University Montreal, Que.
来源
IMMUNOCHEMISTRY | 1968年 / 5卷 / 03期
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0019-2791(68)90076-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The C-terminal heptapeptide, Lys-Glu-Leu-Gly-Tyr-Gln-Gly, of sperm-whale myoglobin was synthesized by the Merrifield solid-phase method. The corresponding monospecific antibodies were eluted with the synthetic peptide from an immunosorbent cosisting of myoglobin coupled to an ethylenemaleic-anhydride copolymer. The purified antibodies were labelled with 131I and their monospecificity was demonstrated by radio-immunodiffusion. © 1968.
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页码:314 / &
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