MEASUREMENT OF CYTOKINE PRODUCTION BY THE MONOCYTIC CELL-LINE MONO MAC-6 USING NOVEL IMMUNORADIOMETRIC ASSAYS FOR INTERLEUKIN-1 BETA AND INTERLEUKIN-6

被引：25

作者：

RAFFERTY, B

MOWER, JA

TAKTAK, YS

POOLE, S

机构：

[1] Division of Endocrinology, National Institute for Biological Standards and Control, Potters Bar, Herts EN 6 3QG, Blanche Lane, South Mimms

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1991年 / 144卷 / 01期

关键词：

INTERLEUKIN-1-BETA; INTERLEUKIN-6; IMMUNORADIOMETRIC ASSAY; MONO MAC-6; PYROGEN TEST;

D O I：

10.1016/0022-1759(91)90232-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Immunoradiometric assays for interleukin-1-beta and interleukin-6 were developed using affinity-purified IgG fractions from antisera initially raised for radioimmunoassay. Capture and detector functions were carried out by the same antibody preparation within each assay. The assays were precise, rapid and 6-8-fold more sensitive than the RIA systems previously employed. IRMAs were used for the initial characterisation of a candidate cell line (Mono Mac 6), under investigation in a 'monocyte test' for pyrogen detection, and permitted rapid and specific determination of the cytokines under stimulatory and inhibitory conditions.

引用

页码：69 / 76

页数：8

共 23 条

[1] 2-SITE ASSAY OF HUMAN GROWTH HORMONE [J].

ADDISON, GM ;

HALES, CN .

HORMONE AND METABOLIC RESEARCH, 1971, 3 (01) :59-&

[2]

BRAKENHOFF JPJ, 1990, J IMMUNOL, V145, P561

[3]

Bristow A F, 1988, Dev Biol Stand, V69, P103

[4] DETERMINATION OF PLASMA CALCITONIN GENE-RELATED PEPTIDE CONCENTRATIONS BY A NEW IMMUNOCHEMILUMINOMETRIC ASSAY IN NORMAL PERSONS AND PATIENTS WITH MEDULLARY-THYROID CARCINOMA AND OTHER NEUROENDOCRINE TUMORS [J].

CARTER, WB ;

TAYLOR, RL ;

KAO, PC ;

HEATH, H .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1991, 72 (02) :327-335

[5]

GAFFNEY EW, 1987, BIOTECHNIQUES, V5, P652

[6] SENSITIVE ELISA FOR INTERLEUKIN-6 - DETECTION OF IL-6 IN BIOLOGICAL-FLUIDS - SYNOVIAL-FLUIDS AND SERA [J].

HELLE, M ;

BOEIJE, L ;

DEGROOT, E ;

DEVOS, A ;

AARDEN, L .

JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 138 (01) :47-56

[7] SELECTIVE ELUTION OF IMMUNO-ADSORBED ANTI- (HUMAN-PROLACTIN) IMMUNOGLOBULINS WITH ENHANCED IMMUNOCHEMICAL PROPERTIES [J].

HODGKINSON, SC ;

LOWRY, PJ .

BIOCHEMICAL JOURNAL, 1982, 205 (03) :535-541

[8] IMMUNORADIOMETRIC VERSUS RADIOIMMUNOASSAY - A COMPARISON USING ALPHA-FETOPROTEIN AS THE MODEL ANALYTE [J].

HUNTER, WM ;

BUDD, PS .

JOURNAL OF IMMUNOLOGICAL METHODS, 1981, 45 (03) :255-273

[9] AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE MEASUREMENT OF HUMAN INTERLEUKIN-6 [J].

IDA, N ;

SAKURAI, S ;

HOSAKA, T ;

HOSOI, K ;

KUNITOMO, T ;

MATSUURA, Y ;

KOHASE, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 133 (02) :279-284

[10] DEXAMETHASONE INHIBITION OF INTERLEUKIN-1 BETA-PRODUCTION BY HUMAN-MONOCYTES - POSTTRANSCRIPTIONAL MECHANISMS [J].

KERN, JA ;

LAMB, RJ ;

REED, JC ;

DANIELE, RP ;

NOWELL, PC .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 81 (01) :237-244

← 1 2 3 →