MOLECULAR-CLONING OF NILE GLYCOPROTEIN AND EVIDENCE FOR ITS CONTINUED EXPRESSION IN MATURE RAT CNS

被引:44
作者
PRINCE, JT [1 ]
ALBERTI, L [1 ]
HEALY, PA [1 ]
NAUMAN, SJ [1 ]
STALLCUP, WB [1 ]
机构
[1] LA JOLLA CANC RES FDN,10901 N TORREY PINES RD,LA JOLLA,CA 92037
关键词
L1; CELL ADHESION MOLECULE; NERVE FIBER TRACT; CDNA CLONING; ANTI-FUSION PROTEIN ANTIBODIES;
D O I
10.1002/jnr.490300315
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The NILE glycoprotein is a rat neuronal cell adhesion molecule which has been reported to be very similar in structure, function, and distribution to the mouse L1 glycoprotein. Here we report the complete nucleotide sequence of the NILE message (5,208 nucleotides) and the deduced amino acid sequence of the NILE polypeptide (1,257 amino acids). The predicted NILE protein is 96% identical to L1 at the amino acid level, confirming that the two molecules are homologues. The sequence information shows that NILE is a transmembrane molecule with an extensive ecto-domain and a much smaller cytoplasmic domain. The extracellular portion of the molecule contains six immunoglobulin C-2 type domains followed by five fibronectin type III repeats. These two structural motifs are characteristic of several other cell adhesion molecules. The cytoplasmic tails of NILE and L1 are identical to each other and distinct from the cytoplasmic regions of all other cell adhesion molecules except Ng-CAM and neuroglian. Several possible sites for phosphorylation are present in the cytoplasmic tail of NILE. Antisera were produced against two NILE-beta-galactosidase fusion proteins containing distinct segments of the NILE polypeptide: the cytoplasmic domain and the segment containing fibronectin type III repeats. Immunoblots with these antisera and Northern blots with a NILE cDNA probe indicate that NILE continues to be expressed in most areas of the mature rat brain. This contradicts previous immunofluorescence data, which suggested that NILE was substantially down-regulated in maturing nerve fiber tracts. This raises the possibility that NILE could be masked in situ by interactions with other cell surface molecules.
引用
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页码:567 / 581
页数:15
相关论文
共 72 条
  • [1] AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303
  • [2] ISOLATION AND NUCLEOTIDE-SEQUENCE OF MOUSE NCAM CDNA THAT CODES FOR A MR 79000 POLYPEPTIDE WITHOUT A MEMBRANE-SPANNING REGION
    BARTHELS, D
    SANTONI, MJ
    WILLE, W
    RUPPERT, C
    CHAIX, JC
    HIRSCH, MR
    FONTECILLACAMPS, JC
    GORIDIS, C
    [J]. EMBO JOURNAL, 1987, 6 (04) : 907 - 914
  • [3] BEASLEY L, 1987, J NEUROSCI, V7, P708
  • [4] DROSOPHILA NEUROGLIAN - A MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY WITH EXTENSIVE HOMOLOGY TO THE VERTEBRATE NEURAL ADHESION MOLECULE L1
    BIEBER, AJ
    SNOW, PM
    HORTSCH, M
    PATEL, NH
    JACOBS, JR
    TRAQUINA, ZR
    SCHILLING, J
    GOODMAN, CS
    [J]. CELL, 1989, 59 (03) : 447 - 460
  • [5] DEMONSTRATION OF IMMUNOCHEMICAL IDENTITY BETWEEN THE NERVE GROWTH FACTOR-INDUCIBLE LARGE EXTERNAL (NILE) GLYCOPROTEIN AND THE CELL ADHESION-L1
    BOCK, E
    RICHTERLANDSBERG, C
    FAISSNER, A
    SCHACHNER, M
    [J]. EMBO JOURNAL, 1985, 4 (11) : 2765 - 2768
  • [6] ISOLATION OF A CDNA CLONE CODING FOR A POSSIBLE NEURAL NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT
    BOULTER, J
    EVANS, K
    GOLDMAN, D
    MARTIN, G
    TRECO, D
    HEINEMANN, S
    PATRICK, J
    [J]. NATURE, 1986, 319 (6052) : 368 - 374
  • [7] NEURAL CELL RECOGNITION MOLECULE F11 - HOMOLOGY WITH FIBRONECTIN TYPE-III AND IMMUNOGLOBULIN TYPE-C DOMAINS
    BRUMMENDORF, T
    WOLFF, JM
    FRANK, R
    RATHJEN, FG
    [J]. NEURON, 1989, 2 (04) : 1351 - 1361
  • [8] STRUCTURE OF THE CHICKEN NEURON-GLIA CELL-ADHESION MOLECULE, NG-CAM - ORIGIN OF THE POLYPEPTIDES AND RELATION TO THE IG SUPERFAMILY
    BURGOON, MP
    GRUMET, M
    MAURO, V
    EDELMAN, GM
    CUNNINGHAM, BA
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 112 (05) : 1017 - 1029
  • [9] CARROLL SB, 1987, DNA CLONING PRACTICA, V3, P89
  • [10] NEURAL CELL-ADHESION MOLECULE - STRUCTURE, IMMUNOGLOBULIN-LIKE DOMAINS, CELL-SURFACE MODULATION, AND ALTERNATIVE RNA SPLICING
    CUNNINGHAM, BA
    HEMPERLY, JJ
    MURRAY, BA
    PREDIGER, EA
    BRACKENBURY, R
    EDELMAN, GM
    [J]. SCIENCE, 1987, 236 (4803) : 799 - 806