FACTORS INFLUENCING THE SEPARATION AND QUANTITATION OF INTACT GLUCOSINOLATES AND DESULPHOGLUCOSINOLATES BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Micellar electrokinetic capillary chromatography methods using cetyltrimethylammonium bromide as a surfactant have been developed for the qualitative and quantitative determination of intact glucosinolates and desulphoglucosinolates. The influence of changes in separation conditions has been investigated. A great number of different intact glucosinolates and desulphoglucosinolates have been used for the development of efficient separation conditions for the closely related, but structurally different, compounds. Repeatability and linearity of the quantitative analyses have been evaluated, and critical parameters have been determined. Rapid and efficient separations are possible for glucosinolates in crude extracts and for mixtures of glucosinolates isolated from seeds and the vegetative parts of plants.