ROLE OF ATP IN THE INTRAMITOCHONDRIAL SORTING OF CYTOCHROME-C(1) AND THE ADENINE-NUCLEOTIDE TRANSLOCATOR

被引：70

作者：

WACHTER, C ^{[1
]}

SCHATZ, G ^{[1
]}

GLICK, BS ^{[1
]}

机构：

[1] BIOCTR, KLINGELBERGSTR 70, CH-4056 BASEL, SWITZERLAND

来源：

EMBO JOURNAL | 1992年 / 11卷 / 13期

关键词：

CONSERVATIVE SORTING; INTERMEMBRANE SPACE; MITOCHONDRIA; PROTEIN IMPORT; STOP TRANSFER;

D O I：

10.1002/j.1460-2075.1992.tb05584.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Import of precursor proteins across the mitochondrial inner membrane requires ATP in the matrix. However, some precursors can still cross the outer membrane in ATP-depleted mitochondria. Here we show that the adenine nucleotide translocator is imported normally into the inner membrane after the matrix has been depleted of ATP. This result supports the earlier suggestion that the translocator inserts into the inner membrane without passing through the matrix. Depletion of matrix ATP also has no detectable effect on the import and maturation of cytochrome c1, which is targeted to the intermembrane space. It thus seems probable that cytochrome c1 does not completely cross the inner membrane during its import pathway.

引用

页码：4787 / 4794

页数：8

共 48 条

[1]

BEASLEY E M, 1992, Current Opinion in Cell Biology, V4, P646, DOI 10.1016/0955-0674(92)90084-P

[2] PHOSPHODIESTER BOND-CLEAVAGE OUTSIDE MITOCHONDRIA IS REQUIRED FOR THE COMPLETION OF PROTEIN IMPORT INTO THE MITOCHONDRIAL MATRIX [J].