SLOW REPAIR OF PYRIMIDINE DIMERS AT P53 MUTATION HOTSPOTS IN SKIN-CANCER

被引：259

作者：

TORNALETTI, S ^{[1
]}

PFEIFER, GP ^{[1
]}

机构：

[1] BECKMAN RES INST CITY HOPE,DUARTE,CA 91010

来源：

SCIENCE | 1994年 / 263卷 / 5152期

关键词：

D O I：

10.1126/science.8128225

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ultraviolet light has been linked with the development of human skin cancers. Such cancers often exhibit mutations in the p53 tumor suppressor gene. Ligation-mediated polymerase chain reaction was used to analyze at nucleotide resolution the repair of cyclobutane pyrimidine dimers along the p53 gene in ultraviolet-irradiated human fibroblasts. Repair rates at individual nucleotides were highly variable and sequence-dependent. Slow repair was seen at seven of eight positions frequently mutated in skin cancer, suggesting that repair efficiency may strongly contribute to the mutation spectrum in a cancer-associated gene.

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页码：1436 / 1438

页数：3

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