ON PROBLEM OF AN ENZYMATIC REGULATION OF DNA SYNTHESIS IN CHLORELLA

被引：24

作者：

WANKA, F

POELS, CLM

机构：

[1] Laboratorium voor Chemische Cytologie, der Universiteit Driehuizerweg, Nijmegen

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 04期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00633.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uridine kinase and thymidylate kinase were investigated in Chlorella cultures synchronized by alternating periods of 16 hours light and 8 hours darkness. The enzymes were activated and stabilized by mercaptoethanol and glutathione. Both enzyme activities increased periodically between the 8th and 18th hour after beginning of the light period. The increase was blocked in the presence of 15 μM actidione. When the light was turned off at the 12th hour the enzyme accumulation ceased after another 90 min. DNA synthesis took place between the 10th and 18th hour and subsequently ceased completely, although high dTMP kinase activities were still observed. The results do not support the hypothesis that DNA synthesis in Chlorella is regulated by thymidylate kinase. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：478 / &

共 15 条

[1]

BERGER CHRISTEL, 1966, FLORA ALLG BOT ZEITUNG [JENA], V157, P211

[2] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].