N-ACYLSARCOSINES AS INHIBITORS OF RESPIRATION AND GLYCOLYSIS AND GLYCOLYTIC ENZYMES

Sodium-N-lauroyl sarcosinate and sodium-N-palmitoyl sarcosinate inhibit the respiration and anaerobic glycolysis of isolated mouse diaphragm. Rabbit heart glucokinase and rabbit muscle fructose-1,6-diphosphate aldolase are also inhibited by SLS and SPS, which appear to act as denaturing agents. In concentrations ranging from 1 × 10-2 m to 3 × 10-2 m for SLS and 2.5 × 10-5 m to 2.5 × 10-4 m for SPS both compounds cause progressive unfolding of rabbit muscle aldolase, as evidenced by the exposure of all the buried sulfhydryl groups; this process is associated with loss of enzymatic activity. © 1969.