SPIN FILTER PERFUSION SYSTEM FOR HIGH-DENSITY CELL-CULTURE - PRODUCTION OF RECOMBINANT URINARY TYPE PLASMINOGEN-ACTIVATOR IN CHO CELLS

被引：82

作者：

AVGERINOS, GC ^{[1
]}

DRAPEAU, D ^{[1
]}

SOCOLOW, JS ^{[1
]}

MAO, J ^{[1
]}

HSIAO, K ^{[1
]}

BROEZE, RJ ^{[1
]}

机构：

[1] COLLABORAT RES INC,2 OAK PK,BEDFORD,MA 01730

来源：

BIO-TECHNOLOGY | 1990年 / 8卷 / 01期

关键词：

D O I：

10.1038/nbt0190-54

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have used a 20 liter stirred tank fermentor, equipped with a 127 mesh ethylene-tetraflouroethylene rotating screen for cell recycle, for the continuous production of recombinant single chain urokinase-type plasminogen activator (rscu-PA) from Chinese hamster ovary (CHO) cells. Viable cell densities between 60 and 74 million per ml were maintained at medium perfusion rates of 3.0 to 4.0 fermentor volumes per day. Cells were retained by the 120 micron nominal opening filter through the formation of clumped cell aggregates of 200 to 60um, which did not foul the filter. In 31 days of culture, a total of 51 grams of rscu-PA were produced in 1, 000 liters of medium. The rscu-PA produced over the course of this continuous culture was purified and characterized both in vitro and in vivo and shown to be comparable to natural scu-PA produced from the transformed human kidney cell line, TCL-598. © 1990 Nature Publishing Group.

引用

页码：54 / 58

页数：5

共 35 条

[1]

AMADOR E, 1963, CLIN CHEM, V9, P391

[2]

AVGERINOS GC, 1988, JAN ENG F C CELL CUL

[3]

BERG GJ, 1988, ANIMAL CELL BIOTECHN, P322

[4]

BEUDING E, 1955, J BIOL CHEM, V215, P507

[5]

BRENNAN A J, 1987, Biotechnology Techniques, V1, P169

[6]

BROEZE RJ, 1988, FIBRINOLYSIS, V2, P65

[7]

BROWN PC, 1988, ANIMAL CELL BIOTECHN, V3, P251

[8] THE LARGE-SCALE CULTIVATION OF MAMMALIAN-CELLS [J].

FEDER, J ;

TOLBERT, WR .

SCIENTIFIC AMERICAN, 1983, 248 (01) :36-&

[9]

FEDER J, 1985, AM BIOTECHNOLOGY JAN, P24

[10]

FRY ETA, 1988, CIRCULATION S, V78, P230

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