ACTIVATION OF PROTHROMBIN BARCELONA - EVIDENCE FOR ACTIVE HIGH MOLECULAR-WEIGHT INTERMEDIATES

被引：20

作者：

RABIET, MJ ^{[1
]}

ELION, J ^{[1
]}

BENAROUS, R ^{[1
]}

LABIE, D ^{[1
]}

JOSSO, F ^{[1
]}

机构：

[1] UER NECKER, DEPT HEMATOL, F-75015 PARIS, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 584卷 / 01期

关键词：

Prothrombin activation; Prothrombin Barcelona; Thrombin;

D O I：

10.1016/0304-4165(79)90236-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prothrombin Barcelona is a varient of human prothrombin. Its activation by factor Xa is characterized by two abnormal features. The first one is the absence of one of the two Xa-catalyzed cleavages, and the second one the generation of a thrombin-like activity responsible for the thrombin-catalyzed cleavages on the prothrombin molecule and for the activity toward small substrates but without clotting activity. The molecular species exhibiting the thrombin-like activity have been characterized Diisopropyl phosphofluoridate incorporation experiments show that upon activation, two high molecular weight intermediates bearing an active site are formed: one has the molecular weight of prothrombin, the other of prethrombin 1. In the presence of hirudin, only the first intermediate is formed due to a single cleavage by factor Xa which is therefore responsible for the unmasking of the active site. © 1979.

引用

页码：66 / 75

页数：10

共 20 条

[1]

ALAGILLE D, 1972, TECHNIQUES HEMATOLOG, P164

[2]

BAJAJ SP, 1973, J BIOL CHEM, V248, P7729

[3] ISOLATION AND PARTIAL CHARACTERIZATION OF A HUMAN PROTHROMBIN VARIANT - PROTHROMBIN BARCELONA [J].