PURIFICATION OF PLASMODIUM-LOPHURAE EXOERYTHROCYTIC MEROZOITES BY AN ION-EXCHANGE COLUMN

被引：7

作者：

HOLLINGDALE, MR

KILEJIAN, A

机构：

[1] Laboratory of Parasitology, Rockefeller University, New York, New York, 10021

来源：

JOURNAL OF PROTOZOOLOGY | 1979年 / 26卷 / 04期

关键词：

DEAE‐cellulose column; electron microscopy; exoerythrocytic merozoites; Plasmodium lophurae; polyacrylamide gel electrophoresis;

D O I：

10.1111/j.1550-7408.1979.tb04207.x

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

SYNOPSIS Exoerythrocytic merozoites of Plasmodium lophurae grown in embryonic turkey brain cells were successfully separated from host cell material by elution from a DEAE‐cellulose column at ionic strength 0.22. Purity of parasite samples was assessed by sodium dodecyl sulphate acrylamide gel electrophoresis and electron microscopy. Increasing the ionic strength gave greater recoveries of merozoites, but host cell contamination increased. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：616 / 619

页数：4

共 10 条

[1] PLASMODIUM - FINE STRUCTURE OF MALARIAL PARASITES [J].