STUDIES ON APPLE PROTOPECTIN .5. STRUCTURAL STUDIES ON ENZYMATICALLY EXTRACTED PECTINS

被引:55
作者
RENARD, CMGC [1 ]
VORAGEN, AGJ [1 ]
THIBAULT, JF [1 ]
PILNIK, W [1 ]
机构
[1] AGR UNIV WAGENINGEN,FOOD CHEM LAB,6700 EV WAGENINGEN,NETHERLANDS
关键词
D O I
10.1016/0144-8617(91)90099-X
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Apple insoluble pectins were extracted by pectolytic enzymes alone and combined with side-chain degrading enzymes or endo-glucanase. The highest yield of pectic material was obtained with the combination pectinlyase plus endo-glucanase, showing the existence of a connection between rhamnogalacturonan and xyloglucan. The extracts were fractionated on Sephacryl S 500 and DEAE Sepharose CL-6B. Methylation analysis of high-molecular-weight material coming from the 'hairy' regions showed the presence of highly branched arabinans and arabinogalactans of type I and II side-chains, and of terminal xylose residues linked to the rhamnogalacturonan backbone.
引用
收藏
页码:137 / 154
页数:18
相关论文
共 39 条
[1]   STRUCTURAL INVESTIGATIONS ON THE NON-STARCHY POLYSACCHARIDES OF APPLES [J].
ASPINALL, GO ;
FANOUS, HK .
CARBOHYDRATE POLYMERS, 1984, 4 (03) :193-214
[2]   APPLE FRUIT PECTIC SUBSTANCES [J].
BARRETT, AJ ;
NORTHCOT.DH .
BIOCHEMICAL JOURNAL, 1965, 94 (03) :617-&
[3]  
De Vries J, 1982, CARBOHYD POLYM, V2, P25
[4]  
De Vries J. A., 1983, CARBOHYD POLYM, V3, P193
[5]  
de Vries J. A., 1981, CARBOHYD POLYM, V1, P117, DOI DOI 10.1016/0144-8617(81)90004-7
[6]  
DEVRIES JA, 1983, CARBOHYD POLYM, V3, P245
[7]   SIMPLIFIED METHOD FOR THE MEASUREMENT OF TOTAL NON-STARCH POLYSACCHARIDES BY GAS - LIQUID-CHROMATOGRAPHY OF CONSTITUENT SUGARS AS ALDITOL ACETATES [J].
ENGLYST, HN ;
CUMMINGS, JH .
ANALYST, 1984, 109 (07) :937-942
[8]  
FRY SC, 1986, ANNU REV PLANT PHYS, V37, P165, DOI 10.1146/annurev.arplant.37.1.165
[9]  
HAKAMORI S, 1964, J BIOCHEM-TOKYO, V55, P205
[10]   AN IMPROVED PROCEDURE FOR THE METHYLATION ANALYSIS OF OLIGOSACCHARIDES AND POLYSACCHARIDES [J].
HARRIS, PJ ;
HENRY, RJ ;
BLAKENEY, AB ;
STONE, BA .
CARBOHYDRATE RESEARCH, 1984, 127 (01) :59-73