STRUCTURE AND EXPRESSION OF GENES FOR A CLASS OF CYSTEINE-RICH PROTEINS OF THE CUTICLE LAYERS OF DIFFERENTIATING WOOL AND HAIR-FOLLICLES

被引:66
作者
MACKINNON, PJ [1 ]
POWELL, BC [1 ]
ROGERS, GE [1 ]
机构
[1] UNIV ADELAIDE,CTR GENE TECHNOL,DEPT BIOCHEM,ADELAIDE,SA 5001,AUSTRALIA
关键词
D O I
10.1083/jcb.111.6.2587
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The major histological components of the hair follicle are the hair cortex and cuticle. The hair cuticle cells encase and protect the cortex and undergo a different developmental program to that of the cortex. We report the molecular characterization of a set of evolutionarily conserved hair genes which are transcribed in the hair cuticle late in follicle development. Two genes were isolated and characterized, one expressed in the human follicle and one in the sheep follicle. Each gene encodes a small protein of 16 kD, containing > 50 cysteine residues, ranging from 31 to 36 mol % cysteine. Their high cysteine content and in vitro expression data identify them as ultra-high-sulfur (UHS) keratin proteins. The predicted proteins are composed almost entirely of cysteine-rich and glycine-rich repeats. Genomic blots reveal that the UHS keratin proteins are encoded by related multigene families in both the human and sheep genomes. Tissue in situ hybridization demonstrates that the expression of both genes is localized to the hair fiber cuticle and occurs at a late stage in fiber morphogenesis.
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页码:2587 / 2600
页数:14
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共 58 条
[1]  
AUBER L, 1950, T R SOC EDINB 1, V52, P191
[2]   OVALBUMIN GENE-SEQUENCE OF PUTATIVE CONTROL REGIONS [J].
BENOIST, C ;
OHARE, K ;
BREATHNACH, R ;
CHAMBON, P .
NUCLEIC ACIDS RESEARCH, 1980, 8 (01) :127-142
[3]   SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].
BENTON, WD ;
DAVIS, RW .
SCIENCE, 1977, 196 (4286) :180-182
[4]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[5]   TRANSCRIPTION TERMINATION AND 3' PROCESSING - THE END IS IN SITE [J].
BIRNSTIEL, ML ;
BUSSLINGER, M ;
STRUB, K .
CELL, 1985, 41 (02) :349-359
[6]  
BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025
[7]   INFLUENCE OF SULPHUR-CONTAINING AMINO ACIDS ON BIOSYNTHESIS OF HIGH-SULPHUR WOOL PROTEINS [J].
BROAD, A ;
GILLESPI.JM ;
REIS, PJ .
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES, 1970, 23 (01) :149-&
[8]  
CHOO KH, 1986, GENE, V46, P277, DOI 10.1016/0378-1119(86)90412-9
[9]  
CREWTHER WG, 1976, 5TH P INT WOOL TEXT, V1, P1
[10]  
DAVIS LG, 1986, BASIC METHODS MOL BI, P244