PREFERENTIAL AND ASYMMETRIC INTERACTION OF LINKER HISTONES WITH 5S DNA IN THE NUCLEOSOME

被引：143

作者：

HAYES, JJ

WOLFFE, AP

机构：

[1] Laboratory of Molecular Embryology, NICHHD, National Institutes of Health, Bethesda

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 14期

关键词：

D O I：

10.1073/pnas.90.14.6415

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We establish that linker histones H1 and H5 bind preferentially to a Xenopus borealis somatic 5S RNA gene associated with an octamer of core histones rather than to naked 5S DNA. This preferential binding requires free linker DNA to either side of the nucleosome core. Incorporation of a single linker histone molecule into the nucleosome protects an additional 20 bp of linker DNA from micrococcal nuclease digestion. This additional DNA is asymmetrically distributed with respect to the nucleosome core. Incorporation of linker histones causes no change to the cleavage of DNA in the nucleosome by hydroxyl radical or DNase I.

引用

页码：6415 / 6419

页数：5

共 37 条

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