A BACILLUS-SUBTILIS LARGE ORF CODING FOR A POLYPEPTIDE HIGHLY SIMILAR TO POLYKETIDE SYNTHASES

被引：48

作者：

SCOTTI, C

PIATTI, M

CUZZONI, A

PERANI, P

TOGNONI, A

GRANDI, G

GALIZZI, A

ALBERTINI, AM

机构：

[1] UNIV PAVIA, DIPARTIMENTO GENET & MICROBIOL, VIA ABBIATEGRASSO 207, I-27100 PAVIA, ITALY

[2] ENIRICERCHE SPA, I-20097 SAN DONATO MILANESE, ITALY

[3] UNIV PAVIA, CTR INTERUNIV STUDIO MACROMOLEC INFORMAZ, I-27100 PAVIA, ITALY

来源：

GENE | 1993年 / 130卷 / 01期

关键词：

GENOMIC SEQUENCING; SECONDARY METABOLISM; ERYA GENES; FATTY ACID SYNTHASE; PROTEIN HOMOLOGY; DELETION MUTANTS;

D O I：

10.1016/0378-1119(93)90347-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The nucleotide (nt) sequence of 13.6 kb of the outG locus of Bacillus subtilis, which maps at approximately 155-degrees between the genetic markers nrdA and polC, was determined. One putative coding sequence was identified corresponding to a large polypeptide of 4427 amino acids (aa). Structural organization at the nt and aa sequence level and extensive similarities of the deduced product, especially to EryA, suggest that the locus is potentially responsible for the synthesis of a polyketide molecule. The locus has been renamed pksX. Comparison of the deduced product with known fatty acid and polyketide synthases (PKS) suggested the presence of beta-ketosynthase, dehydratase, beta-ketoreductase and acyl-carrier protein domains. Preliminary data obtained with deletion mutants indicate that pksX is not an essential gene.

引用

页码：65 / 71

页数：7

共 30 条

[1] THE MULTIFUNCTIONAL 6-METHYLSALICYLIC ACID SYNTHASE GENE OF PENICILLIUM-PATULUM - ITS GENE STRUCTURE RELATIVE TO THAT OF OTHER POLYKETIDE SYNTHASES [J].