INACTIVATION OF PEROXIDASE BY HYDROGEN-PEROXIDE AND ITS PROTECTION BY A REDUCTANT AGENT

被引：164

作者：

ARNAO, MB

ACOSTA, M

DELRIO, JA

GARCIACANOVAS, F

机构：

[1] UNIV MURCIA, DEPT BIOQUIM & BIOL MOLEC, E-30001 MURCIA, SPAIN

[2] UNIV MURCIA, DEPT BIOL VEGETAL FISIOL VEGETAL, E-30001 MURCIA, SPAIN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1038卷 / 01期

关键词：

Enzyme inactivation; Horseadish peroxidase; Hydrogen peroxide; Peroxidase;

D O I：

10.1016/0167-4838(90)90014-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen peroxide, the oxidant substrate of peroxidase, is also an inactivating agent of this enzyme. The reductant substrates protect the enzyme from the inactivating process. A reaction mechanism is proposed, in which two competitive routes exist for Compound I of peroxidase; one catalytic and one inactivating. The analytical solution produced at the end of the reaction supports the proposed mechanism and shows the dependence between the number of turnovers of the enzyme (r) and the ratio of both substrates. © 1990.

引用

页码：85 / 89

页数：5

共 21 条

[1] KINETIC CHARACTERIZATION OF THE INACTIVATION PROCESS OF 2 PEROXIDASE ISOENZYMES IN THE OXIDATION OF INDOLYL-3-ACETIC ACID [J].