OPERATIONAL EFFECTIVENESS FACTORS OF IMMOBILIZED ENZYME-SYSTEMS

被引:24
作者
KASCHE, V [1 ]
KAPUNE, A [1 ]
SCHWEGLER, H [1 ]
机构
[1] UNIV BREMEN,DEPT PHYS,D-2800 BREMEN 33,FED REP GER
关键词
D O I
10.1016/0141-0229(79)90010-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The steady-state and operational effectiveness factors for hydrolytic enzymes immobilized in spherical gel particles have been calculated by the collocation method for a wide range of microenvironmental conditions (given by the Thiele modulus) and macroenvironmental conditions (given by the Sherwood number and the relative substrate content). The operational effectiveness factor is a measure of the ratio of the times required to convert a defined amount of substrate with the same amount of free and immobilized enzyme, respectively. Calculations were made for reactors where the diffusion layers of the different enzyme-containing gel particles do not overlap. The theoretical values were compared with experimental values for stirred reactors with chymotrypsin and trypsin immobilized in spherical particles (Sepharose and Sephadex). Low molecular weight substrates were used. The theoretical and experimental values were found to agree within the experimental error. This demonstrates the predictive capacity of the collocation method in estimating steady-state and operational effectiveness factors for enzyme reactors. The microenvironment and macroenvironment were both found to influence the effectiveness over a wide range of substrate concentrations. However, the macroenvironmental influence is negligible when the Sherwood number of the reactor is larger than ∼50. Then, the diffusion layer thickness is small compared with the dimensions of the enzyme-containing particles. The effectiveness factors calculated here can also be used to predict the performance of continuous stirred tank and plug-flow reactors. © 1979.
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页码:41 / 46
页数:6
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