PURIFICATION AND CHARACTERIZATION OF THE EXTRACELLULAR AND CELL-BOUND LIPASES FROM A PENICILLIUM-CYCLOPIUM VARIETY

被引：20

作者：

DRUET, D ^{[1
]}

ELABBADI, N ^{[1
]}

COMEAU, LC ^{[1
]}

机构：

[1] UDESAM,CHIM BIOL APPL LAB,3 PL VICTOR HUGO,F-13331 MARSEILLE 3,FRANCE

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1992年 / 37卷 / 06期

关键词：

D O I：

10.1007/BF00174840

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new strain of Penicillium cyclopium was found on mouldy Grenoble walnuts. From the culture filtrate, two new extracellular lipases (A- and B-lipase) were isolated and characterized. The mycelium contained one of the two forms of lipase (B-lipase). The specificity of the cell-bound lipase linked to the mycelium was then studied. The enzyme was specific to short-chain homogeneous triacylglycerols; it preferred to attack the o-position rather than the p-position, and showed stereospecificity on the sn-1 position.

引用

页码：745 / 749

页数：5

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