A COLD SHOCK-SYRINGE METHOD FOR THE PREPARATION OF AN AXENIC CULTURE OF THE GIANT COENOCYTIC ALGA VALONIA-VENTRICOSA

被引：2

作者：

NAKANISHI, K ^{[1
]}

SAGA, N ^{[1
]}

机构：

[1] TOKAI UNIV,DEPT MARINE SCI,SHIZUOKA 424,JAPAN

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 75卷 / 02期

关键词：

Algae; -; Bacteriology;

D O I：

10.1016/0922-338X(93)90227-Y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We developed a new method for the preparation of an axenic culture of the giant coenocytic green alga Valonia ventricosa. Aplanospore formation was induced by exposing the organism to cold shock (15-degrees-C) for one day. The aplanospore was then aseptically isolated with a syringe. The aplanospore grown on ESS1 agar medium developed into axenic thalli (2 mm in diameter) after 2 months.

引用

页码：149 / 150

页数：2

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