CDNA CLONING AND EXPRESSION OF 2 NEW MEMBERS OF THE HUMAN LIVER UDP-GLUCURONOSYLTRANSFERASE-2B SUBFAMILY

被引：111

作者：

JIN, CJ ^{[1
]}

MINERS, JO ^{[1
]}

LILLYWHITE, KJ ^{[1
]}

MACKENZIE, PI ^{[1
]}

机构：

[1] FLINDERS UNIV S AUSTRALIA,MED CTR,DEPT CLIN PHARMACOL,BEDFORD PK,SA 5042,AUSTRALIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 01期

关键词：

D O I：

10.1006/bbrc.1993.1847

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two new UDP-glucuronosyltransferase cDNAs, designated UGT2B10 and UGT2B11, encoding 528 amino acid proteins were isolated from a human liver cDNA library. The deduced amino acid sequences of UGTs 2B10 and 2B11 share >76% sequence similarity with other known human liver UGT2B subfamily isoforms and <48% sequence similarity with UGT1 family proteins. COS-7 cells transfected with UGT 2B10 and 2B11 synthesized proteins with respective molecular masses of 49 kDa and 51 kDa. UGT2B11 expressed in COS-7 cells glucuronidated a number of polyhydroxylated estrogens (estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). Despite the screening of more than forty potential substrates, glucuronidation activity was not observed for expressed UGT2B10. © 1993 Academic Press, Inc.

引用

页码：496 / 503

页数：8

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