DEVELOPMENT OF A SPECIES-SPECIFIC POLYMERASE CHAIN-REACTION ASSAY FOR GARDNERELLA-VAGINALIS

被引：19

作者：

VANBELKUM, A

KOEKEN, A

VANDAMME, P

VANESBROECK, M

GOOSSENS, H

KOOPMANS, J

KUIJPERS, J

FALSEN, E

QUINT, W

机构：

[1] SSDZ, CTR DIAGNOST, DEPT MOLEC BIOL, 2600 GA DELFT, NETHERLANDS

[2] UNIV ANTWERP HOSP, DEPT MED MICROBIOL, ANTWERP, BELGIUM

[3] STATE UNIV GHENT, DEPT MICROBIOL, GHENT, BELGIUM

[4] REINIER DE GRAAF HOSP, DEPT GYNAECOL, 2600 GA DELFT, NETHERLANDS

[5] GOTHENBURG UNIV, DEPT CLIN BACTERIOL, S-41346 GOTHENBURG, SWEDEN

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 03期

关键词：

GARDNERELLA VAGINALIS; POLYMERASE CHAIN REACTION; INTERNAL TRANSCRIBED SPACER REGION;

D O I：

10.1006/mcpr.1995.0024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleotide sequence of the region between the 16S and 23S rRNA genes of the facultative anaerobic bacterium Gardnerella vaginalis has been determined, together with the 5' proximal 500 nucleotides of the 23S rRNA gene. Regions suited for the development of specific, probe-confirmable polymerase chain reaction (PCR) assays were selected. PCR assays were evaluated with respect to sensitivity and specificity, the latter in comparison with a number of G. vaginalis reference strains and closely related species like Bifidobacterium spp. In an initial diagnostic study it appeared that the PCR test detected G. vaginalis in 40% of women irrespective of their clinical status. Ten out of 11 patients suffering from bacterial vaginosis as defined on the basis of clinical parameters were carrying G. vaginalis.

引用

页码：167 / 174

页数：8

共 24 条

[1]

Dowson C.G., Hutchison A., Woodford N., Johnson A.P., George R.C., Spratt B.G., Penicillin-resistant Viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of, Streptococcus Pneumoniae. Proceedings of the National Academy of Science USA, 87, pp. 5858-5862, (1990)

[2]

Ehrlich G.D., PCR-based laboratory methods for the detection of the human retroviridae and hepad-naviridae, Pcr-Based Diagnostics for Infectious Disease., pp. 415-416, (1993)

[3]

Ehrlich G.D., Greenberg S., Abbott M., Detection of human T-cell lymphoma/leukemia viruses (HTLV), PCR Protocols: A Guide to Methods and Applications, pp. 325-336, (1990)

[4]

Brisson-Noel A., Gicquel B., Lecossier D., Levy-Frebault V., Nassif X., Hance A.J., Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples, Lancet, 5, pp. 1069-1071, (1989)

[5]

Burstain J.M., Grimprel E., Lukehart S.A., Norgard M.V., Radolf J.D., Sensitive detection of Treponema pallidum by using the polymerase chain reaction, Journal of Clinical Microbiology, 29, pp. 62-69, (1991)

[6]

Demmier G.J., Buffone G.J., Schimbor C.M., May R.A., Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification, Journal of Infectious Diseases, 158, pp. 1177-1184, (1988)

[7]

Isaacman D.J., Zhang Y., Wald E.R., Wadowsky R., Ehrlich G., A sensitive and specific assay utilizing the polymerase chain reaction for the detection of S. Pneumoniae in blood, Pediatric Research, 35, (1994)

[8]

Kwok S., Mack D.H., Mullis K.B., Poiesz B., Ehrlich G., Blair D., Friedman-Kien A., Sninsky J.J., Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection, Journal of Virology, 61, pp. 1690-1694, (1987)

[9]

Morris B.J., Flanagan J.L., McKinnon K.J., Nightingale B.N., (1988). Papillomavirus screening of cervical lavages by polymerase chain reaction, Lancet, 5

[10]

Shankar P., Manjunath N., Lakshmi R., Aditi B., Seth P., Shriniwas S., Identification of Mycobacterium tuberculosis by polymerase chain reaction, Lancet, 335, (1990)

← 1 2 3 →