HIGH-RESOLUTION STRUCTURES OF SINGLE-METAL-SUBSTITUTED CONCANAVALIN-A - THE CO,CA-PROTEIN AT 1.6-ANGSTROM AND THE NI,CA-PROTEIN AT 2.0-ANGSTROM

被引:46
作者
EMMERICH, C
HELLIWELL, JR
REDSHAW, M
NAISMITH, JH
HARROP, SJ
RAFTERY, J
KALB, AJ
YARIV, J
DAUTER, Z
WILSON, KS
机构
[1] UNIV MANCHESTER,DEPT CHEM,OXFORD RD,MANCHESTER M13 9PL,LANCS,ENGLAND
[2] WEIZMANN INST SCI,DEPT STRUCT BIOL,IL-76100 REHOVOT,ISRAEL
[3] UNIV BORDEAUX 1,CRISTALLOG LAB,CNRS,URA 144,F-33405 TALENCE,FRANCE
[4] DESY,EMBL,D-22603 HAMBURG,GERMANY
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷
基金
英国惠康基金;
关键词
D O I
10.1107/S0907444994002143
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The molecular structures of cobalt- and nickel-substituted concanavalin A have been refined at 1.6 and 2.0 angstrom resolution, respectively. Both metal derivatives crystallize in space group I222 with approximate cell dimensions a = 89, b = 87 and c = 63 angstrom and one monomer in the asymmetric unit. The final R factor for Co-substituted concanavalin A is 17.8% for 29211 reflections with F > 1.0 sigma(F) between 8.0 and 1.6 angstrom. For Ni-substituted concanavalin A the final R factor is 15.9% for 16128 reflections with F > 1.0sigma(F) between 8.0 and 2.0 angstrom resolution. Both structures contain a transition-metal binding site and a calcium-binding site but, unlike Cd-substituted concanavalin A, do not have a third metal-binding site. The Co-substituted concanavalin A structure diffracts to the highest resolution of any concanavalin A structure reported to date. A comparison of the structures of Ni-, Co-, Cd-substituted and native concanavalin A gives an indication of coordinate errors, which is a useful baseline for comparisons with saccharide complexes of concanavalin A described in other work. We also give a detailed account of multiple conformations which were found for five side-chain residues.
引用
收藏
页码:749 / 756
页数:8
相关论文
共 24 条
  • [1] BECKER JW, 1975, J BIOL CHEM, V250, P1513
  • [2] BITTIGER H, 1976, CONCANAVALIN A TOOL
  • [3] Brtinger A. T., 1990, X PLOR VERSION 2 1
  • [4] Cotton F.A., 1988, ADV INORGANIC CHEM, V5th, P1385
  • [5] THE STRUCTURE OF THE SACCHARIDE-BINDING SITE OF CONCANAVALIN-A
    DEREWENDA, Z
    YARIV, J
    HELLIWELL, JR
    KALB, AJ
    DODSON, EJ
    PAPIZ, MZ
    WAN, T
    CAMPBELL, J
    [J]. EMBO JOURNAL, 1989, 8 (08) : 2189 - 2193
  • [6] AN X-RAY CRYSTALLOGRAPHIC STUDY OF CONCANAVALIN-A
    GREER, J
    KAUFMAN, HW
    KALB, AJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1970, 48 (02) : 365 - &
  • [7] MANGANESE AND CALCIUM-BINDING SITES OF CONCANAVALIN-A
    HARDMAN, KD
    AGARWAL, RC
    FREISER, MJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) : 69 - 86
  • [8] HARROP SJ, 1993, ACTA CRYSTALLOGR A, V49, pC94
  • [9] HIGASHI T, 1990, PROCESS PROGRAM INDE
  • [10] GRAPHICS MODEL-BUILDING AND REFINEMENT SYSTEM FOR MACROMOLECULES
    JONES, TA
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1978, 11 (AUG) : 268 - 272