EVALUATION OF THE USE OF ELECTROPHORESIS AND CARBON FURNACE ATOMIC-ABSORPTION SPECTROMETRY TO DETERMINE THE COPPER LEVEL IN SEPARATED SERUM-PROTEIN FRACTIONS

被引：8

作者：

TEAPE, J ^{[1
]}

KAMEL, H ^{[1
]}

BROWN, DH ^{[1
]}

OTTAWAY, JM ^{[1
]}

SMITH, WE ^{[1
]}

机构：

[1] UNIV STRATHCLYDE,DEPT PURE & APPL CHEM,GLASGOW G1 1XL,SCOTLAND

来源：

CLINICA CHIMICA ACTA | 1979年 / 94卷 / 01期

关键词：

D O I：

10.1016/0009-8981(79)90180-3

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The results of an evaluation of the use of atomic absorption spectrometry with carbon furnace atomisation to analyse the copper content of individual protein fractions separated by electrophoresis is reported. Albumin copper levels in patients with rheumatoid arthritis were found to be about 1 to 2% of the total serum copper. Both albumin and α2 levels are affected by contamination from residual copper present on the strip. An investigation of this contamination indicated very low blank copper levels in the areas of the strip containing α1 and β proteins. These results, and the results of analysis of copper in the ultrafiltrate from the same serum samples, are combined to define the copper distribution in a group of patients with rheumatoid arthritis. The determinations that can most readily be carried out by this technique are discussed. © 1979.

引用

页码：1 / 8

页数：8

共 10 条

[1] SIGNIFICANCE OF PLASMA COPPER AND CERULOPLASMIN CONCENTRATIONS IN RHEUMATOID-ARTHRITIS [J].

BAJPAYEE, DP .

ANNALS OF THE RHEUMATIC DISEASES, 1975, 34 (02) :162-165

[2] SERUM COPPER LEVELS IN RHEUMATOID-ARTHRITIS [J].

BROWN, DH ;

ELGHOBAREY, A ;

SMITH, WE ;

TEAPE, J .

ANNALS OF THE RHEUMATIC DISEASES, 1978, 37 (04) :391-392

[3]

BROWN DH, ANN RHEUM DIS

[4] MICRODETERMINATION OF COPPER IN PLASMA-PROTEIN FRACTIONS [J].

DELVES, HT .

CLINICA CHIMICA ACTA, 1976, 71 (03) :495-500

[5] DETERMINATION OF GOLD IN SEPARATE PROTEIN-FRACTIONS OF BLOOD-SERUM BY CARBON FURNACE ATOMIC-ABSORPTION SPECTROMETRY [J].

KAMEL, H ;

BROWN, DH ;

OTTAWAY, JM ;

SMITH, WE .

ANALYST, 1977, 102 (1218) :645-657

[6] DETERMINATION OF COPPER IN PLASMA ULTRA-FILTRATE BY ATOMIC-ABSORPTION SPECTROMETRY USING CARBON FURNACE ATOMIZATION [J].

KAMEL, H ;

TEAPE, J ;

BROWN, DH ;

OTTAWAY, JM ;

SMITH, WE .

ANALYST, 1978, 103 (1230) :921-927

[7] ATOMIC-ABSORPTION DETERMINATION OF MANGANESE, COBALT AND COPPER IN WHOLE-BLOOD AND SERUM, WITH A GRAPHITE ATOMIZER [J].

MUZZARELLI, RAA ;

ROCCHETTI, R .

TALANTA, 1975, 22 (08) :683-685

[8] SERUM COPPER AND RELATED VARIABLES IN RHEUMATOID-ARTHRITIS [J].

SCUDDER, PR ;

ALTIMIMI, D ;

MCMURRAY, W ;

WHITE, AG ;

ZOOB, BC ;

DORMANDY, TL .

ANNALS OF THE RHEUMATIC DISEASES, 1978, 37 (01) :67-70

[9]

Sorenson J R, 1976, Ann Rheum Dis, V35, P186, DOI 10.1136/ard.35.2.186

[10] COPPER CHELATES AS POSSIBLE ACTIVE FORMS OF ANTIARTHRITIC AGENTS [J].

SORENSON, JRJ .

JOURNAL OF MEDICINAL CHEMISTRY, 1976, 19 (01) :135-148

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