ARACHIDONIC AND DOCOSAHEXANOIC ACID CONTENT OF BOVINE BRAIN MYELIN - IMPLICATIONS FOR THE PATHOGENESIS OF MULTIPLE-SCLEROSIS

被引：10

作者：

ANSARI, KA ^{[1
]}

SHOEMAN, DW ^{[1
]}

机构：

[1] UNIV MINNESOTA,SCH MED,DEPT NEUROL,MINNEAPOLIS,MN 55455

来源：

NEUROCHEMICAL RESEARCH | 1990年 / 15卷 / 01期

关键词：

arachidonic acid; docosahexanoic acid; Myelin;

D O I：

10.1007/BF00969177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipids were extracted from bovine brain myelin using a mixture of hexane and isopropanol (3:2). Myelin lipids were resolved, using Sep Pak chromatography, into four fractions: Fraction 1 contained neutral lipids, fraction 2, free fatty acids, fraction 3, ethanolamine phospholipids and fraction 4, choline phospholipids. Docosahexanoic (DHA) and arachidonic (AA) acids in these fractions were measured by RPHPLC. Fraction 2 was analyzed directly, the other three fractions were subjected to alkaline hydrolysis before analysis for DHA and AA. DHA and AA were not found in fraction 1. Both DHA and AA were found in fractions 2 and 3. Only AA was consistently found in fraction 4. These results were confirmed by GC. © 1990 Plenum Publishing Corporation.

引用

页码：7 / 11

页数：5

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