METHOD FOR QUANTITATIVE-ANALYSIS OF 16,16-DIMETHYL-PROSTAGLANDIN-E2 FROM PLASMA USING DEUTERATED CARRIER AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY

被引：7

作者：

STEFFENRUD, S

LINCOLN, FH

机构：

[1] KAROLINSKA HOSP,DEPT CHEM,S-10401 STOCKHOLM,SWEDEN

[2] UPJOHN CO,EXPTL CHEM RES,KALAMAZOO,MI 49001

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90118-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The preparation of 3,3,4,4-2H416,16-dimethyl-prostaglandin E2 (PGE2), 16,16-dimethyl-5,6-didehydro-PGE2, and 5,6-3H216,16-dimethyl-PGE2 is described. These compounds have been used for development of a gas-liquid chromatography-mass spectrometry method for quantitative determination of corresponding nondeuterated prostaglandin. The method is based on addition of a known amount of carrier to the sample and after purification and derivation the ratio between the protium and deuterium form is measured in the mass spectrometer. With this technique 40 pg of 16,16-dimethyl-PGE2 can be determined with a precision of ±2.6%. © 1979.

引用

收藏

页码：109 / 117

页数：9

相关论文

共 22 条

[1] SEPARATION OF BILE ACIDS WITH REVERSED PHASE PARTITION CHROMATOGRAPHY [J].

BERGSTROM, S ;

SJOVALL, J .

ACTA CHEMICA SCANDINAVICA, 1951, 5 (9-10) :1267-1270

[2] QUANTITATIVE-DETERMINATION OF MAJOR METABOLITE OF PROSTAGLANDINS-F1ALPHA AND PROSTAGLANDINS-F2ALPHA IN HUMAN URINE BY STABLE ISOTOPE DILUTION AND COMBINED GAS CHROMATOGRAPHY MASS SPECTROMETRY [J].

BRASH, AR ;

BAILLIE, TA ;

CLARE, RA ;

DRAFFAN, GH .

BIOCHEMICAL MEDICINE, 1976, 16 (01) :77-94

[3] NEW METHOD FOR DETERMINATION OF MAJOR METABOLITE OF PROSTAGLANDIN-F2-ALPHA IN HUMAN URINE BASED ON STABLE ISOTOPE DILUTION AND GAS CHROMATOGRAPHY MASS SPECTROMETRY [J].

BRASH, AR ;

DRAFFAN, GH ;

CLARE, RA ;

BAILLIE, TA .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1976, 4 (04) :706-708

[4] PROTECTION OF HYDROXYL GROUPS AS TERT-BUTYLDIMETHYLSILYL DERIVATIVES [J].

COREY, EJ ;

VENKATESWARLU, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1972, 94 (17) :6190-+

[5] GAS CHROMATOGRAPHY-MASS SPECTROMETRY OF O-METHYLOXIME DERIVATIVES OF PROSTAGLANDINS [J].

GREEN, K .

CHEMISTRY AND PHYSICS OF LIPIDS, 1969, 3 (03) :254-&

[6] METHODS FOR QUANTITATIVE-ANALYSIS OF PGF2-ALPHA, PGE2, 9-ALPHA, 11-ALPHA-DIHYDROXY-15-KETO-PROST-5-ENOIC ACID AND 9-ALPHA, 11-ALPHA, 15-TRIHYDROXY-PROST-5-ENOIC ACID FROM BODY-FLUIDS USING DEUTERATED CARRIERS AND GAS CHROMATOGRAPHY MASS SPECTROMETRY [J].

GREEN, K ;

GRANSTRO.E ;

SAMUELSS.B ;

AXEN, U .

ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :434-453

[7] METHOD FOR QUANTITATIVE-ANALYSIS OF PGA2 FROM PLASMA USING DEUTERATED CARRIER AND GAS-LIQUID CHROMATOGRAPHY MASS SPECTROMETRY [J].

GREEN, K ;

STEFFENRUD, S .

ANALYTICAL BIOCHEMISTRY, 1976, 76 (02) :606-614

[8] METABOLISM OF PROSTAGLANDIN-E2 IN RAT [J].

GREEN, K .

BIOCHEMISTRY, 1971, 10 (06) :1072-&

[9]

GREEN K, 1964, J LIPID RES, V5, P117

[10] TERT-BUTYL DIMETHYLSILYL ETHERS AS DERIVATIVES FOR QUALITATIVE ANALYSIS OF STEROIDS AND PROSTAGLANDINS BY GAS-PHASE METHODS [J].

KELLY, RW ;

TAYLOR, PL .

ANALYTICAL CHEMISTRY, 1976, 48 (03) :465-467