TRANSFORMATION OF MYCOBACTERIUM-AURUM BY ELECTROPORATION - THE USE OF GLYCINE, LYSOZYME AND ISONICOTINIC-ACID HYDRAZIDE IN ENHANCING TRANSFORMATION EFFICIENCY

被引：16

作者：

HERMANS, J

BOSCHLOO, JG

DEBONT, JAM

机构：

[1] Division of Industrial Microbiology, Department of Food Science, Agricultural University, Wageningen

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 72卷 / 1-2期

关键词：

Electroporation; Transformation efficiency;

D O I：

10.1016/0378-1097(90)90375-Z

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A transformation procedure for Myobacterium aurum using electroporation was developed and optimized. Effects of glycine and lysozyme treatments were studied, and isonicotinic acid hydrazide was shown to increase transformation efficiency ten-fold. © 1990.

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收藏

页码：221 / 224

页数：4

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