THE USE OF 5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE AND FLOW-CYTOMETRY FOR THE VISUALIZATION OF RESPIRATORY ACTIVITY IN INDIVIDUAL CELLS OF MICROCOCCUS-LUTEUS

被引：84

作者：

KAPRELYANTS, AS

KELL, DB

机构：

[1] UNIV COLL ABERYSTWYTH,DEPT BIOL SCI,ABERYSTWYTH SY23 3DA,DYFED,WALES

[2] RUSSIAN ACAD SCI,BAKH INST BIOCHEM,MOSCOW 117071,RUSSIA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1993年 / 17卷 / 02期

关键词：

TETRAZOLIUM SALT; RESPIRATORY CHAIN; FLUORESCENT FORMAZAN DERIVATIVE; FLOW CYTOMETRY;

D O I：

10.1016/0167-7012(93)90004-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

5-cyano-2,3-ditolyl tetrazolium chloride is a redox dye which may be reduced to a fluorescent formazan derivative. We describe its use, together with flow cytometry, for the visualisation of respiratory activity in individual cells of Micrococcus luteus, and in combination with exogenous NADH for the distinction in a frozen/thawed population of 'injured' cells which have an impaired permeability barrier to the pyridine nucleotide.

引用

页码：115 / 122

页数：8

共 17 条

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