An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

被引：55

作者：

Bresciani, Erica ^{[1
]}

Broadbridge, Elizabeth ^{[1
]}

Liu, Paul P. ^{[1
]}

机构：

[1] NHGRI, Oncogenesis & Dev Sect, NIH, Bethesda, MD 20892 USA

来源：

METHODSX | 2018年 / 5卷

关键词：

Zebrafish; Dissociation; Single cell suspension;

D O I：

10.1016/j.mex.2018.10.009

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing. Published by Elsevier B.V.

引用

页码：1287 / 1290

页数：4