BABESIA EQUI ERYTHROCYTIC STAGE CONTINUOUSLY CULTURED IN AN ENRICHED MEDIUM

被引:49
作者
HOLMAN, PJ
CHIEVES, L
FRERICHS, WM
OLSON, D
WAGNER, GG
机构
[1] Diagnostic Bacteriology Laboratory, National Veterinary Services Lab., United States Dept. of Agriculture, Ames
关键词
D O I
10.2307/3283752
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Babesia equi was continuously cultured through 90 passages in an enriched chemically defined basal medium (HL-1) supplemented with 20% fetal bovine serum and serum replacement factors, including lipid-rich bovine serum albumin, bovine insulin, and human transferrin. Cryopreservation and subsequent recovery of B. equi were easily achieved. Inoculation of a splenectomized and an intact horse with cultured infected erythrocytes resulted in parasitemias and B. equi in vitro reisolation from both animals. In vitro forms of the parasite resembled in vivo forms. After establishment, parasitemias of 10-15% were commonly observed.
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页码:232 / 236
页数:5
相关论文
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