MOLECULAR CHARACTERIZATION OF PSEUDOCERCOSPORELLA-HERPOTRICHOIDES ISOLATES BY AMPLIFICATION OF RIBOSOMAL DNA INTERNAL TRANSCRIBED SPACERS

被引：27

作者：

POUPARD, P ^{[1
]}

SIMONET, P ^{[1
]}

CAVELIER, N ^{[1
]}

BARDIN, R ^{[1
]}

机构：

[1] UNIV LYON 1,ECOL MICROBIENNE SOL LAB,CNRS,URA 1450,F-69622 VILLEURBANNE,FRANCE

来源：

PLANT PATHOLOGY | 1993年 / 42卷 / 06期

关键词：

D O I：

10.1111/j.1365-3059.1993.tb02673.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In order to determine whether a given isolate of Pseudocercosporella herpotrichoides, the cause of eyespot disease of cereals, belongs to the W-type or the R-type, we have developed a test based on the polymerase chain reaction (PCR). Sequences of the internal transcribed spacer (ITS) region of the ribosomal genes were determined for three different isolates of each pathotype. Primers complementary to variable sequences were specifically chosen to amplify differentially DNA from W- and R-types. Specific reaction conditions of the PCR assay were experimentally determined by testing four isolates of each type that exhibit a wide range of sensitivity to fungicides.

引用

页码：873 / 881

页数：9

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